Chlamydia trachomatis can cause a wide range of diseases, involving the eyes, genital tract, rectum and other organs, and may be transmitted from mothers to babies. Gonorrhea is a major STD in China, caused by Neisseria gonorrhoeae infection, and characterized by short latency and strong infectivity. Serious complications and sequelae can occur if it not cured in time. Ureaplasma urealyticum can cause urogenital tract infection, which is most closely related to female reproductive health. 80% of pregnant women have Ureaplasma urealyticum in their genital tract, which may infect infants via the placenta and lead to premature delivery, stillbirth or neonatal infection during delivery, causing respiratory tract infection. In addition, Ureaplasma urealyticum can also cause infertility. Therefore, rapid detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Ureaplasma urealyticum is conducive to epidemic surveillance and development of effective prevention and control measure.