The kit is an in vitro nucleic acid amplification test for the quantification/existence of Human Hepatitis C Virus (HCV) RNA in human serum or plasma. It is intended for use as an aid in diagnosing an HCV infection and observing drug efficacy.

By applying real-time fluorescence quantitative PCR technology, this test utilizes 2 pairs of specific primers which are designed to target at a conserved sequence of HCV-RNA, 2 specific fluorescence probes, accompanied with RT-qPCR mix, to achieve quantitative detection of HCV-RNA through fluorescent signal changes. The PCR detection system uses an internal control, which monitors the presence of PCR inhibitors in test specimens by detecting whether the internal control signal is normal, to avoid a false negative result.